Detection of Salmonella spp. survival and virulence in poultry feed by targeting the hilA gene
نویسندگان
چکیده
منابع مشابه
Detection of Salmonella spp contamination of carcasses slaughtered in poultry abattoir in Mashhad, Iran
A total of 60 neck skin swab samples were taken from 12 different broiler flocks after the chilling stage of processing at a commercial broiler slughtering facility in Mashhad. The presence of Salmonella was assessed by conventional culture method and confirmed by using poly O and poly H antiserum in serotyping. PCR amplification of invA gene as a specific method for detection of Salmonella w...
متن کاملIdentification of Salmonella spp. and Salmonella typhimurium by a multiplex PCR-based assay from poultry carcasses in Mashhad- Iran
Poultry meat has been identified as one of the principal foodborne sources of Salmonella. In this preliminary study the prevalence of Salmonella spp. and its typhimurium serovar contamination of broiler carcasses, were determined. Using the rinse test method, numbers of 60 samples, representing 20 broiler flocks, were collected from poultry carcasses after the chilling stage in the processing l...
متن کاملDetection of Virulence Gene (inva) in Salmonella Isolated from Meat and Poultry Products
Detection of pathogenic Salmonella isolated from meat and poultry products by detecting virulence invA gene using PCR technique.A total of100 meat and poultry products samples were collected from shops and supermarkets; including 50 meat products samples and 50 poultry products. Salmonella was detected by conventional isolation methods and then PCR was carried out to detectinvA gene in isolated...
متن کاملIsolation and Detection of Virulence factors of Salmonella typhimurium and Salmonella enteritidis
Plasmids play an important role in the virulence and antibiotic resistance of Salmonella. Salmonella carry plasmids having different size and number. Plasmid profile analysis of single isolates of Salmonella typhimurium and Salmonella enteritidis revealed the presence of 4- plasmid profiles. Salmonella serovars produce several Type III secretions including Sop-E and Sop –B. Soap E is detected b...
متن کاملExpression of the hilA Salmonella typhimurium gene in a poultry Salm. enteritidis isolate in response to lactate and nutrients.
Pathogens express virulence genes in response to the combination of environmental conditions present in the host environment. The crop is the first gastrointestinal environment encountered in birds. However, feed withdrawal alters the crop environment resulting in an increased pH, and decreased concentrations of lactate, glucose and amino acids compared with unmoulted birds. Salmonella enteriti...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2011
ISSN: 1364-5072
DOI: 10.1111/j.1365-2672.2011.05054.x